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Comparison of serological methods for the diagnostic of Caprine arthritis-encephalitis (CAE) in Rio de Janeiro, Brazil BJM
Cortez-Moreira,Madelayne; Oelemann,Walter M.R.; Lilenbaum,Walter.
Serum samples of 562 goats were tested for Caprine arthritis-encephalitis using agar gel immunodiffusion test (AGID) and enzyme linked immunosorbent assay (ELISA). Seventy-nine samples (14.1%) were reactive by both testes but 141 (25.1%) were reactive only by ELISA. ELISA, when compared to AGID, presented 100% sensitivity and 70.8% specificity.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Caprine arthritis-encephalitis; CAE; Goats; ELISA; AGID.
Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822005000100010
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Improvement of an in-house ELISA for bovine paratuberculosis serology in Brazil BJM
Marassi,Carla Dray; Fonseca,Leila de Souza; Ristow,Paula; Ferreira,Rachel; Lilenbaum,Walter; Oelemann,Walter M.R..
Paratuberculosis (Johne's disease) is a chronic enteritis in cattle caused by infection with Mycobacterium avium subsp. paratuberculosis (Map). In Brazil, few reports describe the isolation of the organism. The gold standard test for Map is its isolation from tissues or feces. Moreover, bacterial growth is slow and test results are available only after three to five months of incubation Furthermore, shedding of bacilli at levels detectable by fecal culture is irregular and does not occur during the early stages of infection, which compromises the sensitivity of this methodology. The most common immunological tests to identify Map infection are complement fixation test (CFT), agar gel immunodiffusion (AGID) and enzyme-linked immunoassay (ELISA). In Brazil,...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Paratuberculosis; Cattle; ELISA; Serology; Diagnosis.
Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822005000200004
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Sensitivity of AMPLICOR MTB on direct detection of Mycobacterium tuberculosis in smear-negative specimens from outpatients in Rio de Janeiro BJM
Anjos Filho,Luciano dos; Oelemann,Walter M.R.; Barreto,Clara E.N.; Kritski,Afranio Lineu; Fonseca,Leila de Souza.
We evaluated the performance of the AMPLICOR MTB assay for detection of M. tuberculosis in clinical specimens obtained from 98 smear-negative tuberculous patients (43 with positive and 55 with negative culture results). The sensitivity of the AMPLICOR MTB was 44.9% (44/98) and that of culture was 43.8% (43/98). No significant difference was observed between the results obtained by AMPLICOR MTB and by culture. We conclude that amplification assays could be used for testing smear-negative specimens because a rapid diagnosis will benefit those patients.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Tuberculosis; AMPLICOR MTB; Smear-negative specimens.
Ano: 2002 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822002000200013
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